Human soluble interleukin-1 receptor I,IL-1sRI ELISA kit

Human soluble interleukin-1 receptor I,IL-1sRI ELISA kit

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Mfr.No.

CSB-E04611h

Sensitivity

0.039 ng/ml

Detection Range

0.156 ng/ml-10 ng/ml

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

interleukin 1 receptor, type I, CD121A, D2S1473, IL-1R-alpha, IL1R, IL1RA, P80, OTTHUMP00000161344, antigen CD121a, interleukin 1 receptor alpha, type I, interleukin receptor 1

Recovery

The recovery of mouse TGF-?1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-99
EDTA plasma (n=4)	96	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TGF-?1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	99
	Range %	94-104
1:2	Average %	89
	Range %	83-97
1:4	Average %	92
	Range %	87-97
1:8	Average %	92
	Range %	87-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
50	2.654	2.636	2.645	2.515
25	2.318	2.455	2.387	2.257
12.5	1.756	1.886	1.821	1.691
6.25	0.997	1.001	0.999	0.869
3.12	0.542	0.512	0.527	0.397
1.56	0.301	0.309	0.305	0.175
0.78	0.228	0.238	0.233	0.103
0	0.129	0.131	0.130	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P14778

Applications	ELISA
Research Area	IL1R1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504381	CSB-E04611h	96T	$595	Add to Cart
BHE10504381	CSB-E04611h	5x96T	$2856	Add to Cart
BHE10504381	CSB-E04611h	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.