Human Soluble Cluster of Differentiation 80 (sCD80) ELISA Kit

Human Soluble Cluster of Differentiation 80 (sCD80) ELISA Kit

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Mfr.No.

CSB-E15768h

Sensitivity

31.25 pg/mL

Detection Range

125 pg/mL-8000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

CD80 molecule, CD28LG, CD28LG1, LAB7, B-lymphocyte activation antigen B7, CD80 antigen, CD80 antigen (CD28 antigen ligand 1, B7-1 antigen), costimulatory factor CD80, costimulatory molecule variant IgV-CD80

Recovery

The recovery of human TPM4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	82-90
EDTA plasma (n=4)	94	90-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human TPM4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	100
	Range %	97-103
1:2	Average %	85
	Range %	81-89
1:4	Average %	91
	Range %	85-97
1:8	Average %	99
	Range %	95-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1500	2.557	2.597	2.577	2.398
750	1.916	1.936	1.926	1.747
375	1.278	1.298	1.288	1.109
187.5	0.798	0.742	0.770	0.591
94	0.481	0.499	0.490	0.311
47	0.367	0.355	0.361	0.182
23.5	0.264	0.268	0.266	0.087
0	0.178	0.179	0.179

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P33681

Applications	ELISA
Research Area	CD80

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504375	CSB-E15768h	96T	$595	Add to Cart
BHE10504375	CSB-E15768h	5x96T	$2856	Add to Cart
BHE10504375	CSB-E15768h	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.