Human Soluble Cluster of differentiation 40 ligand,sCD40L ELISA Kit

Human Soluble Cluster of differentiation 40 ligand,sCD40L ELISA Kit

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Mfr.No.

CSB-E04716h

Sensitivity

0.078 ng/mL

Detection Range

0.312 ng/mL-20 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

CD40 ligand, RP3-527F8.3, CD154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39, hCD40L, CD40 antigen ligand, T-B cell-activating molecule, TNF-related activation protein, tumor necrosis factor (ligand) superfa

Recovery

The recovery of bovine ?-EP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	95-104
EDTA plasma (n=4)	92	88-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine ?-EP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-99
1:2	Average %	84
	Range %	80-88
1:4	Average %	92
	Range %	88-97
1:8	Average %	90
	Range %	85-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
3000	1.701	1.612	1.657	1.559
1500	1.376	1.458	1.417	1.319
750	1.113	1.104	1.109	1.011
375	0.832	0.809	0.821	0.723
187.5	0.524	0.560	0.542	0.444
93.75	0.367	0.359	0.363	0.265
46.88	0.264	0.251	0.258	0.160
0	0.099	0.097	0.098	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P29965

Applications	ELISA
Research Area	CD40LG

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10504373	CSB-E04716h	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.