Human soluble cluster of differentiation 146,sCD146 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E11336h

Alternative Names

soluble cluster of differentiation 146,sCD146

Reactivity

Human

Detection Range

1.25 ng/mL-80 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human CLDN5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	95-106
EDTA plasma (n=4)	95	89-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CLDN5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-97
1:2	Average %	97
	Range %	92-102
1:4	Average %	100
	Range %	94-107
1:8	Average %	92
	Range %	87-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

0.31 ng/mL

Limit Of Detection

450 nm

UniProt ID	P43121
Antigen	soluble cluster of differentiation 146,sCD146

Applications

ELISA

Research Area

sCD146

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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