Human soluble cluster of differentiation 14,sCD14 ELISA Kit

Mfr.No.

CSB-E13199h

Alternative Names

CD14 molecule, CD14 antigen, monocyte differentiation antigen CD14, CD14 molecule, CD14 antigen, monocyte differentiation antigen CD14

Reactivity

Human

Detection Range

0.234 ng/mL-15 ng/mL

Sample Type

Serum, Cell culture supernatants, Plasma, Urine, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse FAS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	95-107
EDTA plasma (n=4)	100	89-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse FAS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	104
	Range %	97-108
1:2	Average %	106
	Range %	97-110
1:4	Average %	94
	Range %	85-97
1:8	Average %	100
	Range %	92-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.965	2.848	2.907	2.737
20	2.658	2.592	2.625	2.455
10	2.166	2.079	2.123	1.953
5	1.502	1.478	1.490	1.320
2.5	0.832	0.844	0.838	0.668
1.25	0.551	0.543	0.547	0.377
0.625	0.329	0.319	0.324	0.154
0	0.172	0.168	0.170	?

Species

Homo sapiens

Sensitivity

0.229 ng/mL

Limit Of Detection

450 nm

UniProt ID	P08571
Antigen	CD14 molecule

Applications

ELISA

Research Area

CD14

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504368	CSB-E13199h	96T	$495	Add to Cart
BHE10504368	CSB-E13199h	5x96T	$2376	Add to Cart
BHE10504368	CSB-E13199h	10x96T	$4702.50	Add to Cart

Human soluble cluster of differentiation 14,sCD14 ELISA Kit

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