Human soluble cluster of differentiation 14,sCD14 ELISA Kit

Mfr.No.

CSB-E13199h

Sensitivity

0.229 ng/mL

Detection Range

0.234 ng/mL-15 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

CD14 molecule, CD14 antigen, monocyte differentiation antigen CD14, CD14 molecule, CD14 antigen, monocyte differentiation antigen CD14

Recovery

The recovery of mouse FAS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	95-107
EDTA plasma (n=4)	100	89-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse FAS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	104
	Range %	97-108
1:2	Average %	106
	Range %	97-110
1:4	Average %	94
	Range %	85-97
1:8	Average %	100
	Range %	92-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P08571

Applications	ELISA
Research Area	CD14

Storage	Store at 2-8°C. Please refer to protocol.

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