Human Sodium/potassium-transporting ATPase subunit alpha-1(ATP1A1) ELISA kit

Mfr.No.

CSB-EL002322HU

Alternative Names

ATPase, Na+/K+ transporting, alpha 1 polypeptide, MGC3285, MGC51750, Na+, K+ ATPase alpha subunit, Na+/K+ -ATPase alpha 1 subunit, Na+/K+ ATPase 1, Na, K-ATPase, alpha-A catalytic polypeptide, Na,K-ATPase alpha-1 subunit, Na,K-ATPase catalytic subunit a

Reactivity

Human

Detection Range

15.6 pg/mL-1000 pg/mL

Sample Type

Tissue homogenates, Cell lysates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human PDGF-AA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	84-94
EDTA plasma (n=4)	104	95-110

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human PDGF-AA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	97
	Range %	90-102
1:2	Average %	100
	Range %	92-104
1:4	Average %	92
	Range %	85-96
1:8	Average %	94
	Range %	85-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.329	2.368	2.349	2.192
10	1.695	1.576	1.636	1.479
5	0.988	0.953	0.971	0.814
2.5	0.651	0.647	0.649	0.492
1.25	0.365	0.355	0.360	0.203
0.625	0.257	0.248	0.253	0.096
0.312	0.205	0.211	0.208	0.051
0	0.159	0.155	0.157	?

Species

Homo sapiens

Sensitivity

3.9 pg/mL

Limit Of Detection

450 nm

UniProt ID	P05023
Antigen	ATPase, Na+/K+ transporting, alpha 1 polypeptide

Applications

ELISA

Research Area

ATP1A1

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504360	CSB-EL002322HU	96T	$695	Add to Cart
BHE10504360	CSB-EL002322HU	5x96T	$3336	Add to Cart
BHE10504360	CSB-EL002322HU	10x96T	$6602.50	Add to Cart

Human Sodium/potassium-transporting ATPase subunit alpha-1(ATP1A1) ELISA kit

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