JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
[email protected]

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / ELISA Kit

Human Sodium- and chloride-dependent glycine transporter 1 (SLC6A9) ELISA Kit

SKU : BHE13702806

Glycine transport is mediated by 2 sodium-dependent carriers, GLYT1 and GLYT2, that have distinct tissue distributions. While the 3-prime sequences of these 2 cDNAs were identical, the 5-prime noncoding regions and the N termini were completely different. GLYT1b was found only in the white matter of the CNS, while GLYT1a was found in the gray matter of the CNS as well as in macrophages and mast cells in peripheral tissues. Tissue-specific alternative splicing or alternative promoter usage from a single gene resulted in 2 mRNA products encoding similar but distinct glycine transporters. The anatomic distribution of GLYT1a mRNA supported the emerging status of glycine as a supraspinal neurotransmitter and suggested that glycine may function as a chemical messenger outside the CNS

Continuing the saving: $50 Off All ELISA Kits Offer Extended!

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Request More Infos

Abbkine Scientific Co., Ltd.

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	KTE60590
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Sodium- and chloride-dependent glycine transporter 1 (SLC6A9) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC6A9.
Alternative Names	SLC6A9; DKFZp547A1118; GLYT1; sodium- and chloride-dependent glycine transporter 1; solute carrier family 6 member 9
Detection Method	Colorimetric
Kit Components	Human Sodium- and chloride-dependent glycine transporter 1 microplate Human Sodium- and chloride-dependent glycine transporter 1 standard Human Sodium- and chloride-dependent glycine transporter 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Sodium- and chloride-dependent glycine transporter 1 (SLC6A9) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC6A9. No significant cross-reactivity or interference between Human SLC6A9 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Glycine transport is mediated by 2 sodium-dependent carriers, GLYT1 and GLYT2, that have distinct tissue distributions. While the 3-prime sequences of these 2 cDNAs were identical, the 5-prime noncoding regions and the N termini were completely different. GLYT1b was found only in the white matter of the CNS, while GLYT1a was found in the gray matter of the CNS as well as in macrophages and mast cells in peripheral tissues. Tissue-specific alternative splicing or alternative promoter usage from a single gene resulted in 2 mRNA products encoding similar but distinct glycine transporters. The anatomic distribution of GLYT1a mRNA supported the emerging status of glycine as a supraspinal neurotransmitter and suggested that glycine may function as a chemical messenger outside the CNS
Notes	This Human Sodium- and chloride-dependent glycine transporter 1 (SLC6A9) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC6A9 in samples. An antibody specific for SLC6A9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC6A9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC6A9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC6A9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	6536

Applications	ELISA
Applications Notes	This Human Sodium- and chloride-dependent glycine transporter 1 (SLC6A9) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC6A9 in samples. An antibody specific for SLC6A9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC6A9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC6A9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC6A9 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

No Description Available.

Have you used this product?

Rate it now..!!

Login to Review

SKU	Supplier No.	Size	Your price	Quantity
BHE13702806	KTE60590	48 T	$339		Add to Cart
BHE13702806	KTE60590	96 T	$589		Add to Cart
BHE13702806	KTE60590	96 Tx5	$2629		Add to Cart
BHE13702806	KTE60590	96 Tx50	$24569		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2024 Biohippo. All rights reserved.