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Home / ELISA Kit

Human Sodium- and chloride-dependent creatine transporter 1 (SLC6A8) ELISA Kit

SKU : BHE13702807

The creatine-phosphocreatine shuttle has important functions in the temporal and spatial maintenance of the energy supply to skeletal and cardiac muscle. Muscle cells do not synthesize creatine, but take it up via a special sodium-dependent transporter, the creatine transporter (SLC6A8). In mouse skin, they found high amounts of cytosolic brain CK (CKB) coexpressed with lower amounts of ubiquitous mitochondrial CK (CKMT1B), both mainly localized in suprabasal layers of the dermis, different cell types of hair follicles, sebaceous glands, and the subcutaneous panniculus carnosus muscle. Except for sebaceous glands, these cells also expressed CRT. Ckb and Crt were upregulated about 3-fold immediately after wounding of mouse skin, whereas the amount of Ckmt1b increased 10 to 15 days after wounding.

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Mfr.No.	KTE60591
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Sodium- and chloride-dependent creatine transporter 1 (SLC6A8) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC6A8.
Alternative Names	SLC6A8; CRT; CRTR; CT1; MGC87396; creatine transporter SLC6A8; sodium- and chloride-dependent creatine transporter 1; solute carrier family 6 member 8
Detection Method	Colorimetric
Kit Components	Human Sodium- and chloride-dependent creatine transporter 1 microplate Human Sodium- and chloride-dependent creatine transporter 1 standard Human Sodium- and chloride-dependent creatine transporter 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Sodium- and chloride-dependent creatine transporter 1 (SLC6A8) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC6A8. No significant cross-reactivity or interference between Human SLC6A8 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The creatine-phosphocreatine shuttle has important functions in the temporal and spatial maintenance of the energy supply to skeletal and cardiac muscle. Muscle cells do not synthesize creatine, but take it up via a special sodium-dependent transporter, the creatine transporter (SLC6A8). In mouse skin, they found high amounts of cytosolic brain CK (CKB) coexpressed with lower amounts of ubiquitous mitochondrial CK (CKMT1B), both mainly localized in suprabasal layers of the dermis, different cell types of hair follicles, sebaceous glands, and the subcutaneous panniculus carnosus muscle. Except for sebaceous glands, these cells also expressed CRT. Ckb and Crt were upregulated about 3-fold immediately after wounding of mouse skin, whereas the amount of Ckmt1b increased 10 to 15 days after wounding.
Notes	This Human Sodium- and chloride-dependent creatine transporter 1 (SLC6A8) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC6A8 in samples. An antibody specific for SLC6A8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC6A8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC6A8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC6A8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	6535

Applications	ELISA
Applications Notes	This Human Sodium- and chloride-dependent creatine transporter 1 (SLC6A8) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC6A8 in samples. An antibody specific for SLC6A8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC6A8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC6A8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC6A8 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702807	KTE60591	48 T	$339		Add to Cart
BHE13702807	KTE60591	96 T	$589		Add to Cart
BHE13702807	KTE60591	96 Tx5	$2629		Add to Cart
BHE13702807	KTE60591	96 Tx50	$24569		Add to Cart

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