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Home / ELISA Kit

Human Small proline-rich protein 2B (SPRR2B) ELISA Kit

SKU : BHE13702566

SPRR genes encode a novel class of polypeptides called small proline-rich proteins that are strongly induced during differentiation of human epidermal keratinocytes in vitro and in vivo. SPRR genes constitute a distinct class of cornified envelope precursor proteins.
Gibbs et al. (1993) concluded that there are 7 genes for the SPRR2 subfamily of small proline-rich proteins, including SPRR2B.Using DNA microarray analysis, Zimmermann et al. (2005) identified Sprr2a and Sprr2b as allergen-induced genes in a mouse model of experimental asthma.By analysis of human-rodent hybrid cell lines and FISH, Gibbs et al. (1993) determined that all SPRR genes, including SPRR2B, are closely linked within a 300-kb DNA segment on chromosome 1q21-q22.

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Mfr.No.	KTE60349
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Small proline-rich protein 2B (SPRR2B) ELISA Kit employs a two-site sandwich ELISA to quantitate SPRR2B.
Alternative Names	SPRR2B; OTTHUMP00000015752
Detection Method	Colorimetric
Kit Components	Human Small proline-rich protein 2B microplate Human Small proline-rich protein 2B standard Human Small proline-rich protein 2B detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Small proline-rich protein 2B (SPRR2B) ELISA Kit has high sensitivity and excellent specificity for detection of Human SPRR2B. No significant cross-reactivity or interference between Human SPRR2B and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	SPRR genes encode a novel class of polypeptides called small proline-rich proteins that are strongly induced during differentiation of human epidermal keratinocytes in vitro and in vivo. SPRR genes constitute a distinct class of cornified envelope precursor proteins. Gibbs et al. (1993) concluded that there are 7 genes for the SPRR2 subfamily of small proline-rich proteins, including SPRR2B.Using DNA microarray analysis, Zimmermann et al. (2005) identified Sprr2a and Sprr2b as allergen-induced genes in a mouse model of experimental asthma.By analysis of human-rodent hybrid cell lines and FISH, Gibbs et al. (1993) determined that all SPRR genes, including SPRR2B, are closely linked within a 300-kb DNA segment on chromosome 1q21-q22.
Notes	This Human Small proline-rich protein 2B (SPRR2B) ELISA Kit employs a two-site sandwich ELISA to quantitate SPRR2B in samples. An antibody specific for SPRR2B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPRR2B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPRR2B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPRR2B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	6701

Applications	ELISA
Applications Notes	This Human Small proline-rich protein 2B (SPRR2B) ELISA Kit employs a two-site sandwich ELISA to quantitate SPRR2B in samples. An antibody specific for SPRR2B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPRR2B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPRR2B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPRR2B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702566	KTE60349	48 T	$339		Add to Cart
BHE13702566	KTE60349	96 T	$589		Add to Cart
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BHE13702566	KTE60349	96 Tx50	$24569		Add to Cart

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