Human Small Dense Low Density Lipoprotein(sLDL)ELISA Kit

Human Small Dense Low Density Lipoprotein(sLDL)ELISA Kit

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Mfr.No.

CSB-E13350h

Alternative Names

Small Dense Low Density Lipoprotein(sLDL)

Reactivity

Human

Detection Range

0.312 nmol/mL-20 nmol/mL

Sample Type

Tissue homogenates, Serum, Plasma, Saliva, Urine

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of mouse CDH5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	92-108
EDTA plasma (n=4)	94	90-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CDH5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	92
	Range %	88-96
1:200	Average %	95
	Range %	91-98
1:400	Average %	100
	Range %	96-105
1:800	Average %	94
	Range %	87-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
15	2.513	2.413	2.463	2.359
7.5	1.698	1.598	1.648	1.544
3.75	0.970	0.940	0.955	0.851
1.88	0.499	0.489	0.494	0.390
0.94	0.326	0.316	0.321	0.217
0.47	0.217	0.207	0.212	0.108
0.235	0.147	0.145	0.146	0.042
0	0.105	0.103	0.104	?

Species

Homo sapiens

Sensitivity

0.078 nmol/mL

Limit Of Detection

450 nm

Antigen	Small Dense Low Density Lipoprotein(sLDL)

Applications

ELISA

Research Area

sLDL

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	The effect of nutritional intervention on the lipid profile and dietary intake of adolescents with juvenile systemic lupus erythematosus: a randomized, controlled trial da Silva SGL.et al,Lupus,Jan 1

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SKU	Supplier No.	Size	Your price
BHE10504349	CSB-E13350h	96T	$790	Add to Cart
BHE10504349	CSB-E13350h	5x96T	$3792	Add to Cart
BHE10504349	CSB-E13350h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.