Human Small Dense Low Density Lipoprotein(sLDL)ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E13350h

Sensitivity

0.078 nmol/mL

Detection Range

0.312 nmol/mL-20 nmol/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

Small Dense Low Density Lipoprotein(sLDL)

Recovery

The recovery of mouse CDH5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	92-108
EDTA plasma (n=4)	94	90-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CDH5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	92
	Range %	88-96
1:200	Average %	95
	Range %	91-98
1:400	Average %	100
	Range %	96-105
1:800	Average %	94
	Range %	87-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	sLDL

Storage	Store at 2-8°C. Please refer to protocol.

Reference	The effect of nutritional intervention on the lipid profile and dietary intake of adolescents with juvenile systemic lupus erythematosus: a randomized, controlled trial da Silva SGL.et al,Lupus,Jan 1

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