Human Smad3/MADH3(Mothers Against Decapentaplegic Homolog 3) ELISA Kit

Mfr.No.

EH2148

Alternative Names

Smad3 ELISA Kit, MADH3 ELISA Kit, JV15-2 ELISA Kit, SMAD family member 3 ELISA Kit, hMAD-3 ELISA Kit, hSMAD3 ELISA Kit, MAD homolog 3 ELISA Kit, mad protein homolog ELISA Kit, MAD ELISA Kit, mothers against decapentaplegic homolog 3 ELISA Kit, MAD ELISA Kit, mothers against decapentaplegic homolog 3 ELISA Kit, Drosophila ELISA Kit, mad3 ELISA Kit, Mad3 ELISA Kit, MADH3mad homolog JV15-2 ELISA Kit, mothers against decapentaplegic homolog 3 ELISA Kit, Mothers against DPP homolog 3 ELISA Kit, SMA- and MAD-related protein 3 ELISA Kit, SMAD family member 3HSPC193 ELISA Kit, SMAD ELISA Kit, mothers against DPP homolog 3 ELISA Kit, SMAD ELISA Kit, mothers against DPP homolog 3 ELISA Kit, Drosophila ELISA Kit, Smad3 ELISA Kit

Reactivity

Human

Detection Method

Double Antibody

Detection Range

0.625-40ng/ml

Sample Type

Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of Smad3/MADH3 and the recovery rates were calculated by comparing the measured value to the expected amount of Smad3/MADH3 in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	86-101	95
EDTA plasma(n=5)	89-105	96
heparin plasma(n=5)	86-102	92

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Smad3/MADH3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	91-101%	92-103%	88-100%
EDTA plasma(n=5)	94-101%	85-97%	85-99%
heparin plasma(n=5)	80-83%	89-100%	82-92%

Sensitivity

< 0.375ng/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

Smad3/MADH3/JV15-2/SMAD family member 3/hMAD-3/hSMAD3/MAD homolog 3/mad protein homolog/MAD, mothers against decapentaplegic homolog 3/MAD, mothers against decapentaplegic homolog 3(Drosophila)/mad3/Mad3/MADH3mad homolog JV15-2/mothers against decapentaplegic homolog 3/Mothers against DPP homolog 3/SMA- and MAD-related protein 3/SMAD family member 3HSPC193/SMAD, mothers against DPP homolog 3/SMAD, mothers against DPP homolog 3(Drosophila)/Smad3

Notes

For Research Use Only

UniProt ID	P84022
Antigen	Smad3/MADH3

Applications	ELISA
Applications Notes	Smad3/MADH3 ELISA Kit allows for the in vitro quantitative determination of Smad3/MADH3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10805562	EH2148	96T	$400		Add to Cart

Human Smad3/MADH3 (Mothers Against Decapentaplegic Homolog 3) ELISA Kit

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