Human Smad1/MADH1 (Mothers Against Decapentaplegic Homolog 1) CLIA Kit

Mfr.No.

E-CL-H0636

Sensitivity

0.09 ng/mL

Detection Range

0.16-10 ng/mL

Reactivity

Human

Assay Duration

3.5h

Assay Type

Sandwich

Description

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human Smad1/MADH1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Smad1/MADH1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Smad1/MADH1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human Smad1/MADH1. You can calculate the concentration of Human Smad1/MADH1 in the samples by comparing the RLU value of the samples to the standard curve.

Detection Method

Chemiluminescence

Recovery

80%-120%

Linearity

Samples were spiked with high concentrations of Human Smad1/MADH1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	85-97	86-98	93-108
1:2	Average (%)	92	92	99
1:4	Range (%)	95-109	100-118	89-103
1:4	Average (%)	103	108	94
1:8	Range (%)	87-98	94-110	96-110
1:8	Average (%)	92	101	102
1:16	Range (%)	92-108	91-104	91-108
1:16	Average (%)	99	97	98

Specificity

This kit recognizes Human Smad1/MADH1 in samples.No significant cross-reactivity or interference between Human Smad1/MADH1 and analogues was observed

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Precision

Both intra-CV and inter-CV are < 10%.

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Usage Notes

This ELISA kit applies to the in vitro quantitative determination of Human Smad1/MADH1 concentrations in Serum, plasma and other biological fluids.

UniProt ID	Q15797

Applications	CLIA
Research Area	Cancer, Epigenetics and Nuclear Signaling, Signal transduction, Stem cells

Storage	2-8°C/-20°C,6 months
Shipping	Ice bag

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SKU	Supplier No.	Size	Your price	Quantity
BHE10300552	E-CL-H0636	96T	$682		Add to Cart
BHE10300552	E-CL-H0636	48T	$546		Add to Cart

Human Smad1/MADH1 (Mothers Against Decapentaplegic Homolog 1) CLIA Kit

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