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Home / ELISA Kit

Human Slit homolog 1 protein (SLIT1) ELISA Kit

SKU : BHE13702798

The SLIT1 cDNA encodes a 1,534-amino acid polypeptide with 43.5% similarity to the Drosophila 'slit' protein. Northern blot analysis revealed that the human SLIT1 gene was expressed as a major 8.4- and a minor 5.9-kb transcript primarily in the brain. SLIT2 and SLIT3 mRNAs were primarily expressed in the spinal cord and thyroid, respectively. In situ hybridization studies indicated that the rat Slit1 mRNA was specifically expressed in the neurons of fetal and adult forebrains. These data suggested that the SLIT genes form an evolutionarily conserved group in vertebrates and invertebrates, and that the mammalian SLIT proteins may participate in the formation and maintenance of the nervous and endocrine systems by protein-protein interactions.

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Mfr.No.	KTE60582
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Slit homolog 1 protein (SLIT1) ELISA Kit employs a two-site sandwich ELISA to quantitate SLIT1.
Alternative Names	SLIT1; RP11-175O19.2; MEGF4; MGC164811; SLIL1; SLIT3; Slit-1; slit homolog 1
Detection Method	Colorimetric
Kit Components	Human Slit homolog 1 protein microplate Human Slit homolog 1 protein standard Human Slit homolog 1 protein detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Slit homolog 1 protein (SLIT1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLIT1. No significant cross-reactivity or interference between Human SLIT1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The SLIT1 cDNA encodes a 1,534-amino acid polypeptide with 43.5% similarity to the Drosophila 'slit' protein. Northern blot analysis revealed that the human SLIT1 gene was expressed as a major 8.4- and a minor 5.9-kb transcript primarily in the brain. SLIT2 and SLIT3 mRNAs were primarily expressed in the spinal cord and thyroid, respectively. In situ hybridization studies indicated that the rat Slit1 mRNA was specifically expressed in the neurons of fetal and adult forebrains. These data suggested that the SLIT genes form an evolutionarily conserved group in vertebrates and invertebrates, and that the mammalian SLIT proteins may participate in the formation and maintenance of the nervous and endocrine systems by protein-protein interactions.
Notes	This Human Slit homolog 1 protein (SLIT1) ELISA Kit employs a two-site sandwich ELISA to quantitate SLIT1 in samples. An antibody specific for SLIT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLIT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLIT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLIT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	6585

Applications	ELISA
Applications Notes	This Human Slit homolog 1 protein (SLIT1) ELISA Kit employs a two-site sandwich ELISA to quantitate SLIT1 in samples. An antibody specific for SLIT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLIT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLIT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLIT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702798	KTE60582	48 T	$339		Add to Cart
BHE13702798	KTE60582	96 T	$589		Add to Cart
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BHE13702798	KTE60582	96 Tx50	$24569		Add to Cart

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