Human SLAM family member 7(SLAMF7) ELISA kit

Human SLAM family member 7(SLAMF7) ELISA kit

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Mfr.No.

CSB-EL021369HU

Alternative Names

SLAM family member 7, 19A, CD319, CRACC, CS1, 19A24 protein, CD2-like receptor activating cytotoxic cells, novel LY9 (lymphocyte antigen 9) like protein

Reactivity

Human

Detection Range

78 pg/mL-5000 pg/mL

Sample Type

Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat IFN-? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-96
EDTA plasma (n=4)	96	90-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat IFN-? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-91
1:2	Average %	92
	Range %	85-97
1:4	Average %	99
	Range %	94-104
1:8	Average %	103
	Range %	99-108

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.087	2.099	2.093	1.916
1000	1.595	1.574	1.585	1.408
500	1.087	1.096	1.092	0.915
250	0.672	0.695	0.684	0.507
125	0.491	0.457	0.474	0.297
62.5	0.376	0.351	0.364	0.187
31.25	0.287	0.299	0.293	0.116
0	0.175	0.178	0.177	?

Species

Homo sapiens

Sensitivity

19.5 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q9NQ25
Antigen	SLAM family member 7

Applications

ELISA

Research Area

SLAMF7

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504344	CSB-EL021369HU	96T	$695	Add to Cart
BHE10504344	CSB-EL021369HU	5x96T	$3336	Add to Cart
BHE10504344	CSB-EL021369HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.