Human SLAM/CD150 ELISA Kit PicoKine®

Mfr.No.

EK1321

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.008	0.050	0.099	0.208	0.401	0.794	1.409	2.065

Description

Human SLAM/CD150 ELISA Kit PicoKine® (96 Tests). Quantitate Human SLAMF1 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Signaling lymphocytic activation molecule;CDw150;IPO-3;CD150;SLAMF1;SLAM;

Molecular Weight

37231 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: A21-K236

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human SLAM

Subcellular Localization

Cell membrane; Single-pass type I membrane protein. Present on the surface of B-cells and T-cells.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	230	1313	5541	247	1409	5429
Standard deviation	11.27	64.33	277.05	14.82	77.49	271.45
CV (%)	4.9%	4.9%	5%	6%	5.5%	5%

Protein Function

High-affinity self-ligand important in biional T- cell to B-cell stimulation. SLAM-induced signal-transduction events in T-lymphocytes are different from those in B-cells. Two modes of SLAM signaling are likely to exist: one in which the inhibitor SH2D1A acts as a negative regulator and another in which protein-tyrosine phosphatase 2C (PTPN11)-dependent signal transduction operates.

Background

Signaling lymphocytic activation molecule is a protein that in humans is encoded by the SLAMF1 gene. It belongs to the immunoglobulin gene superfamily. This gene is mapped to 1q23.3. It has found that SLAM is constitutively expressed on peripheral blood memory T cells, T-cell clones, immature thymocytes and a proportion of B cells, and is rapidly induced on naive T cells after activation. In MV-resistant cell lines, infection with clinical MV and expression of SLAM, but not CD46, caused cytopathic effects (CPE). The expression of SLAM on activated B and T lymphocytes correlates with the pathology of MV infection in humans and monkeys, in which lymphoid organs are the chief sites of MV replication and the binding of MV to SLAM may impair the signaling functions of SLAM in lymphocyte activation and inhibit Th0/Th1 cytokine production, thereby promoting Th2 cytokine production. It has reported that antibody-mediated ligation of SLAM on thymocytes triggered a protein tyrosine phosphorylation signal in T cells in a SAP-dependent manner. This signal also involved SHIP, the adaptor molecules DOK2, DOK1, and SHC and RASGAP.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	230	211	205	246	223	16.17	7.2%
Sample 2	1313	1290	1409	1294	1326	48.41	3.6%
Sample 3	5541	5036	5297	5586	5365	219.47	4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	6504
Gene Name	SLAMF1
UniProt ID	Q13291
Protein Name	Signaling lymphocytic activation molecule
Tissue Specificity	Constitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes and a proportion of B-cells, and is rapidly induced on naive T-cells after activation.
Sequence	Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Applications	ELISA
Research Area	Cell Type Markers,Hematopoietic Progenitors,Host-Virus Interaction,Immunology,Interspecies Interaction,Microbiology,Stem Cells,Surface Molecules

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21000923	EK1321	96 wells/kit	$499		Add to Cart

Human SLAM/CD150 ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human SLAM/CD150 ELISA Kit PicoKine®

Rate it now..!!