JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
[email protected]

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / ELISA Kit

Human Ski-like protein (SKIL) ELISA Kit

SKU : BHE13702864

The mouse Sno gene expresses 2 isoforms, SnoN and SnoN2, that are different from each other in a location downstream of the site of alternative splicing previously described in the human SNO gene. SnoN2 is missing a 138-bp coding segment present in mouse SnoN and human SNON. TGFB induces activation and nuclear translocation of SMAD2, SMAD3, and SMAD4. SMAD3 causes degradation of SnoN, allowing a SMAD2/SMAD4 complex to activate TGFB target genes. To initiate a negative feedback mechanism that permits a precise and timely regulation of TGFB signaling, TGFB also induces an increased expression of SnoN at a later stage, which in turn binds to SMAD heteromeric complexes and shuts off TGFB signaling.

Continuing the saving: $50 Off All ELISA Kits Offer Extended!

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Request More Infos

Abbkine Scientific Co., Ltd.

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	KTE60648
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Ski-like protein (SKIL) ELISA Kit employs a two-site sandwich ELISA to quantitate SKIL.
Alternative Names	SKIL; SNO; SnoA; SnoI; SnoN; SKI-like; ski-related oncogene snoN
Detection Method	Colorimetric
Kit Components	Human Ski-like protein microplate Human Ski-like protein standard Human Ski-like protein detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Ski-like protein (SKIL) ELISA Kit has high sensitivity and excellent specificity for detection of Human SKIL. No significant cross-reactivity or interference between Human SKIL and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The mouse Sno gene expresses 2 isoforms, SnoN and SnoN2, that are different from each other in a location downstream of the site of alternative splicing previously described in the human SNO gene. SnoN2 is missing a 138-bp coding segment present in mouse SnoN and human SNON. TGFB induces activation and nuclear translocation of SMAD2, SMAD3, and SMAD4. SMAD3 causes degradation of SnoN, allowing a SMAD2/SMAD4 complex to activate TGFB target genes. To initiate a negative feedback mechanism that permits a precise and timely regulation of TGFB signaling, TGFB also induces an increased expression of SnoN at a later stage, which in turn binds to SMAD heteromeric complexes and shuts off TGFB signaling.
Notes	This Human Ski-like protein (SKIL) ELISA Kit employs a two-site sandwich ELISA to quantitate SKIL in samples. An antibody specific for SKIL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySKIL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SKIL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SKIL bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	6498

Applications	ELISA
Applications Notes	This Human Ski-like protein (SKIL) ELISA Kit employs a two-site sandwich ELISA to quantitate SKIL in samples. An antibody specific for SKIL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySKIL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SKIL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SKIL bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

No Description Available.

Have you used this product?

Rate it now..!!

Login to Review

SKU	Supplier No.	Size	Your price	Quantity
BHE13702864	KTE60648	48 T	$339		Add to Cart
BHE13702864	KTE60648	96 T	$589		Add to Cart
BHE13702864	KTE60648	96 Tx5	$2629		Add to Cart
BHE13702864	KTE60648	96 Tx50	$24569		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2024 Biohippo. All rights reserved.