Matrices listed below were spiked with certain level of SIRT7 and the recovery rates were calculated by comparing the measured value to the expected amount of SIRT7 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-101
97
EDTA plasma(n=5)
89-101
95
heparin plasma(n=5)
91-96
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SIRT7 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
SIRT7(NAD-dependent deacetylase sirtuin-7)/SIR2L7/Regulatory protein SIR2 homolog 7/SIR2-like protein 7
Notes
For Research Use Only
UniProt ID
Q9NRC8
Antigen
SIRT7
Applications
ELISA
Applications Notes
SIRT7 ELISA Kit allows for the in vitro quantitative determination of SIRT7 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human SIRT7(NAD-dependent deacetylase sirtuin-7) ELISA Kit