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Home / ELISA Kit

Human Signal transducer and activator of transcription 3 (STAT3) ELISA Kit

SKU : BHE13702594

STAT3, a transcription factor for acute phase response genes, acts as an adaptor molecule in signal transduction from the type I interferon receptor. They found that it binds to a conserved sequence in the cytoplasmic tail of the IFNAR1 chain of the receptor and undergoes interferon-dependent tyrosine phosphorylation. The p85 regulatory subunit of phosphatidylinositol 3-kinase, which activates a series of serine kinases, was found to bind to phosphorylated STAT3 and subsequently to undergo tyrosine phosphorylation. The authors concluded that STAT3 acts as an adaptor to couple another signaling pathway to the interferon receptor.In many human cancers and transformed cell lines, STAT3 is persistently activated, and in cell culture, active STAT3 is either required for transformation, enhances transformation, or blocks apoptosis.

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Mfr.No.	KTE60377
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Signal transducer and activator of transcription 3 (STAT3) ELISA Kit employs a two-site sandwich ELISA to quantitate STAT3.
Alternative Names	STAT3; APRF; FLJ20882; HIES; MGC16063; DNA-binding protein APRF; acute-phase response factor; signal transducer and activator of transcription 3
Detection Method	Colorimetric
Kit Components	Human Signal transducer and activator of transcription 3 microplate Human Signal transducer and activator of transcription 3 standard Human Signal transducer and activator of transcription 3 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Signal transducer and activator of transcription 3 (STAT3) ELISA Kit has high sensitivity and excellent specificity for detection of Human STAT3. No significant cross-reactivity or interference between Human STAT3 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	STAT3, a transcription factor for acute phase response genes, acts as an adaptor molecule in signal transduction from the type I interferon receptor. They found that it binds to a conserved sequence in the cytoplasmic tail of the IFNAR1 chain of the receptor and undergoes interferon-dependent tyrosine phosphorylation. The p85 regulatory subunit of phosphatidylinositol 3-kinase, which activates a series of serine kinases, was found to bind to phosphorylated STAT3 and subsequently to undergo tyrosine phosphorylation. The authors concluded that STAT3 acts as an adaptor to couple another signaling pathway to the interferon receptor.In many human cancers and transformed cell lines, STAT3 is persistently activated, and in cell culture, active STAT3 is either required for transformation, enhances transformation, or blocks apoptosis.
Notes	This Human Signal transducer and activator of transcription 3 (STAT3) ELISA Kit employs a two-site sandwich ELISA to quantitate STAT3 in samples. An antibody specific for STAT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySTAT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STAT3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STAT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	6774

Applications	ELISA
Applications Notes	This Human Signal transducer and activator of transcription 3 (STAT3) ELISA Kit employs a two-site sandwich ELISA to quantitate STAT3 in samples. An antibody specific for STAT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySTAT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STAT3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STAT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702594	KTE60377	48 T	$339		Add to Cart
BHE13702594	KTE60377	96 T	$589		Add to Cart
BHE13702594	KTE60377	96 Tx5	$2629		Add to Cart
BHE13702594	KTE60377	96 Tx50	$24569		Add to Cart

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