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Home / ELISA Kit

Human Signal transducer and activator of transcription 2 (STAT2) ELISA Kit

SKU : BHE13702593

Signal transducer and activator of transcription 2 is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. In response to interferon (IFN), this protein forms a complex with STAT1 and IFN regulatory factor family protein p48 (ISGF3G), in which this protein acts as a transactivator, but lacks the ability to bind DNA directly.
Transcription adaptor P300/CBP (EP300/CREBBP) has been shown to interact specifically with this protein, which is thought to be involved in the process of blocking IFN-alpha response by adenovirus.

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Mfr.No.	KTE60376
Description	This Human Signal transducer and activator of transcription 2 (STAT2) ELISA Kit employs a two-site sandwich ELISA to quantitate STAT2.
Alternative Names	STAT2; ISGF-3; MGC59816; P113; STAT113; interferon alpha induced transcriptional activator; signal transducer and activator of transcription 2
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Signal transducer and activator of transcription 2 microplate Human Signal transducer and activator of transcription 2 standard Human Signal transducer and activator of transcription 2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Signal transducer and activator of transcription 2 (STAT2) ELISA Kit has high sensitivity and excellent specificity for detection of Human STAT2. No significant cross-reactivity or interference between Human STAT2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Signal transducer and activator of transcription 2 is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. In response to interferon (IFN), this protein forms a complex with STAT1 and IFN regulatory factor family protein p48 (ISGF3G), in which this protein acts as a transactivator, but lacks the ability to bind DNA directly. Transcription adaptor P300/CBP (EP300/CREBBP) has been shown to interact specifically with this protein, which is thought to be involved in the process of blocking IFN-alpha response by adenovirus.
Notes	This Human Signal transducer and activator of transcription 2 (STAT2) ELISA Kit employs a two-site sandwich ELISA to quantitate STAT2 in samples. An antibody specific for STAT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySTAT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STAT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STAT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	6773
Antigen	STAT2
Tag	STAT2
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Signal transducer and activator of transcription 2 (STAT2) ELISA Kit employs a two-site sandwich ELISA to quantitate STAT2 in samples. An antibody specific for STAT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySTAT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STAT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STAT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702593	KTE60376	48 T	$339		Add to Cart
BHE13702593	KTE60376	96 T	$589		Add to Cart
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BHE13702593	KTE60376	96 Tx50	$24569		Add to Cart

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