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Home / ELISA Kit

Human Signal-regulatory protein beta-2 (SIRPB2) ELISA Kit

SKU : BHE13702871

Signal-regulatory protein gamma is a protein encoded by this gene is a member of the signal-regulatory protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. Alternatively spliced transcript variants encoding different isoforms have been described.
The 354-amino acid protein shares approximately 75% sequence identity with SIRPB1. It possesses a putative signal sequence, a long extracellular domain, a transmembrane region, a short intracellular domain, and no catalytic domain. RT-PCR analysis detected expression of SIRPG at varying levels in all tissues tested.

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Mfr.No.	KTE60655
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Signal-regulatory protein beta-2 (SIRPB2) ELISA Kit employs a two-site sandwich ELISA to quantitate SIRPB2.
Alternative Names	SIRPB2; RP4-776F14.1; PTPN1L; PTPNS1L3; dJ776F14.2; protein tyrosine phosphatase; non-receptor type 1-like; protein tyrosine phosphatase; non-receptor type substrate 1-like 3
Detection Method	Colorimetric
Kit Components	Human Signal-regulatory protein beta-2 microplate Human Signal-regulatory protein beta-2 standard Human Signal-regulatory protein beta-2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Signal-regulatory protein beta-2 (SIRPB2) ELISA Kit has high sensitivity and excellent specificity for detection of Human SIRPB2. No significant cross-reactivity or interference between Human SIRPB2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Signal-regulatory protein gamma is a protein encoded by this gene is a member of the signal-regulatory protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. Alternatively spliced transcript variants encoding different isoforms have been described. The 354-amino acid protein shares approximately 75% sequence identity with SIRPB1. It possesses a putative signal sequence, a long extracellular domain, a transmembrane region, a short intracellular domain, and no catalytic domain. RT-PCR analysis detected expression of SIRPG at varying levels in all tissues tested.
Notes	This Human Signal-regulatory protein beta-2 (SIRPB2) ELISA Kit employs a two-site sandwich ELISA to quantitate SIRPB2 in samples. An antibody specific for SIRPB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySIRPB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SIRPB2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SIRPB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	284759

Applications	ELISA
Applications Notes	This Human Signal-regulatory protein beta-2 (SIRPB2) ELISA Kit employs a two-site sandwich ELISA to quantitate SIRPB2 in samples. An antibody specific for SIRPB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySIRPB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SIRPB2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SIRPB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702871	KTE60655	48 T	$339		Add to Cart
BHE13702871	KTE60655	96 T	$589		Add to Cart
BHE13702871	KTE60655	96 Tx5	$2629		Add to Cart
BHE13702871	KTE60655	96 Tx50	$24569		Add to Cart

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