Human Signal Peptide - Cub and EGF-like Domain-containing Protein 1

Mfr.No.

E3142Hu

Description

This sandwich kit is for the accurate quantitative detection of human Signal Peptide, CUB and EGF-like Domain-containing Protein 1 (also known as SCUBE1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

SCUBE 1;SCUBE1;Signal Peptide, CUB and EGF-like domain-containing protein 1

Reactivity

Human

Detection Range

1-400ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

107910

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.55ng/ml

Background

Could function as an adhesive molecule and its matrix bound and soluble fragments may play a critical role in vascular biology.

Gene ID	80274
UniProt ID	Q8IWY4
Antigen	SCUBE1

Research Area

Cardiovascular

Protocol

These standard curves of E3142Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
240ng/ml	2.304	2.306	2.249
	2.308
120ng/ml	1.554	1.423	1.366
	1.293
60ng/ml	0.909	0.857	0.8
	0.806
30ng/ml	0.497	0.518	0.461
	0.539
15ng/ml	0.296	0.291	0.233
	0.285
0ng/ml	0.06	0.057	0
	0.055

Precision

We measured random samples of E3142Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	155.5	5.69	3.7
Intra-Assay	2	18	81.9	5.64	6.9
Intra-Assay	3	18	38.6	2.6	6.7

Storage	2-8°C

Reference	The diagnostic value of SCUBE1 in unstable angina pectoris patients Journal: Eurasian J Emerg Med 2016; 15: 167-71

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104674	E3142Hu	48T	$320		Add to Cart
BHE12104674	E3142Hu	96T	$458		Add to Cart

Human Signal Peptide - Cub and EGF-like Domain-containing Protein 1 - SCUBE1 ELISA Kit

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