Human Signal-induced Proliferation-associated Protein 1

Mfr.No.

E4739Hu

Sensitivity

4.19ng/L

Detection Range

7-1500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Signal-induced Proliferation-associated Protein 1 (also known as SIPA1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

GTPase-activating protein Spa-1;p130 SPA-1;Signal-induced proliferation-associated protein 1;SIPA 1;SIPA1;Sipa-1

Molecular Weight

112149

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E4739Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	197.1	10.8	5.5
Intra-Assay	2	18	357.5	12.76	3.6
Intra-Assay	3	18	316.1	16.6	5.3

Background

GTPase activator for the nuclear Ras-related regulatory proteins Rap1 and Rap2 in vitro, converting them to the putatively inactive GDP-bound state (PubMed: 9346962). Affects cell cycle progression (By similarity).

Protocol

These standard curves of E4739Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800ng/L	2.261	2.252	2.225
	2.243
400ng/L	1.446	1.432	1.404
	1.418
200ng/L	0.696	0.677	0.649
	0.658
100ng/L	0.419	0.375	0.348
	0.332
50ng/L	0.197	0.163	0.135
	0.128
0ng/L	0.034	0.027	0
	0.021

Gene ID	6494
UniProt ID	Q96FS4

Research Area	Cell Biology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106027	E4739Hu	48T	$320		Add to Cart
BHE12106027	E4739Hu	96T	$458		Add to Cart

Human Signal-induced Proliferation-associated Protein 1 - SIPA1 ELISA Kit

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