Human Sialidase-1 - NEU1 ELISA Kit

Mfr.No.

E0785Hu

Alternative Names

Acetylneuraminyl hydrolase;G9 sialidase;Lysosomal sialidase;N-acetyl-alpha-neuraminidase 1;NEU 1;NEU1;Sialidase-1

Reactivity

Human

Detection Range

0.1-40ng/mL

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

45467

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.045ng/mL

Background

Catalyzes the removal of sialic acid (N-acetylneuraminic acid) moieties from glycoproteins and glycolipids. To be active, it is strictly dependent on its presence in the multienzyme complex. Appears to have a preference for alpha 2-3 and alpha 2-6 sialyl linkage.

Gene ID	4758
UniProt ID	Q99519
Antigen	NEU1

Research Area

Signal Transduction

Protocol

These standard curves of E0785Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/mL	2.341	2.396	2.329
	2.451
12ng/mL	1.219	1.235	1.168
	1.251
6ng/mL	0.763	0.774	0.707
	0.786
3ng/mL	0.483	0.465	0.398
	0.447
1.5ng/mL	0.24	0.243	0.176
	0.246
0ng/mL	0.071	0.067	0
	0.063

Precision

We measured random samples of E0785Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	4.2	0.2	4.9
Intra-Assay	2	18	9	0.5	5.6
Intra-Assay	3	18	18	0.56	3.1

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102464	E0785Hu	48T	$320		Add to Cart
BHE12102464	E0785Hu	96T	$458		Add to Cart

Human Sialidase-1 - NEU1 ELISA Kit

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