Human Sialic Acid-binding Ig-like Lectin 8

Mfr.No.

E6173Hu

Sensitivity

0.023ng/mL

Detection Range

0.05-20ng/mL

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

SAF2;SAF-2;Sialic acid-binding Ig-like lectin 8;Sialoadhesin family member 2;SIGLEC8;SIGLEC-8;SIGLEC8L

Molecular Weight

54042

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E6173Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	7.6	0.27	3.61
Intra-Assay	2	18	2.64	0.14	5.35
Intra-Assay	3	18	5.75	0.27	4.69

Background

Sialic acid-binding Ig-like lectin 8 is a protein that in humans is encoded by the SIGLEC8 gene. This gene is located on chromosome 19q13.4, about 330 kb downstream of the SIGLEC9 gene. Within the siglec family of transmembrane proteins, Siglec-8 belongs to the CD33-related siglec subfamily, a subfamily that has undergone rapid evolution.

Protocol

These standard curves of E6173Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/mL	2.383	2.391	2.338
	2.398
6ng/mL	1.327	1.276	1.224
	1.226
3ng/mL	0.689	0.757	0.704
	0.826
1.5ng/mL	0.505	0.459	0.406
	0.412
0.75ng/mL	0.279	0.286	0.233
	0.293
0ng/mL	0.062	0.053	0
	0.043

Gene ID	27181
UniProt ID	Q9NYZ4

Research Area	Immunology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107535	E6173Hu	48T	$320		Add to Cart
BHE12107535	E6173Hu	96T	$458		Add to Cart

Human Sialic Acid-binding Ig-like Lectin 8 - SIGLEC8 ELISA Kit

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