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Home / ELISA Kit

Human Sialic acid-binding Ig-like lectin 10 (SIGLEC10) ELISA Kit

SKU : BHE13702878

By searching an EST database for sequences resembling CD33 (SIGLEC3) and screening a spleen cDNA library, Munday et al. (2001) isolated a cDNA encoding SIGLEC10. Sequence analysis predicted that the 697-amino acid SIGLEC10 protein contains a signal peptide, an N-terminal V-set Ig-like domain and 4 C2-set Ig-like domains, 5 potential N-linked glycosylation sites, a transmembrane region, and a 126-residue cytoplasmic tail with 3 putative ITIMs. Northern blot analysis detected a major 3.0-kb SIGLEC10 transcript, with highest levels in spleen, lymph node, blood leukocytes, and appendix. Little or no expression was observed in pancreas, thyroid, and testis. Flow cytometric analysis demonstrated eosinophil-specific expression of SIGLEC10, but at a lower level than that of SIGLEC8.

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Mfr.No.	KTE60662
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Sialic acid-binding Ig-like lectin 10 (SIGLEC10) ELISA Kit employs a two-site sandwich ELISA to quantitate SIGLEC10.
Alternative Names	SIGLEC10; MGC126774; PRO940; SIGLEC-10; SLG2; sialic acid binding Ig-like lectin 10 Ig-like lectin 7; siglec-like gene 2
Detection Method	Colorimetric
Kit Components	Human Sialic acid-binding Ig-like lectin 10 microplate Human Sialic acid-binding Ig-like lectin 10 standard Human Sialic acid-binding Ig-like lectin 10 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Sialic acid-binding Ig-like lectin 10 (SIGLEC10) ELISA Kit has high sensitivity and excellent specificity for detection of Human SIGLEC10. No significant cross-reactivity or interference between Human SIGLEC10 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	By searching an EST database for sequences resembling CD33 (SIGLEC3) and screening a spleen cDNA library, Munday et al. (2001) isolated a cDNA encoding SIGLEC10. Sequence analysis predicted that the 697-amino acid SIGLEC10 protein contains a signal peptide, an N-terminal V-set Ig-like domain and 4 C2-set Ig-like domains, 5 potential N-linked glycosylation sites, a transmembrane region, and a 126-residue cytoplasmic tail with 3 putative ITIMs. Northern blot analysis detected a major 3.0-kb SIGLEC10 transcript, with highest levels in spleen, lymph node, blood leukocytes, and appendix. Little or no expression was observed in pancreas, thyroid, and testis. Flow cytometric analysis demonstrated eosinophil-specific expression of SIGLEC10, but at a lower level than that of SIGLEC8.
Notes	This Human Sialic acid-binding Ig-like lectin 10 (SIGLEC10) ELISA Kit employs a two-site sandwich ELISA to quantitate SIGLEC10 in samples. An antibody specific for SIGLEC10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySIGLEC10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SIGLEC10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SIGLEC10 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	89790

Applications	ELISA
Applications Notes	This Human Sialic acid-binding Ig-like lectin 10 (SIGLEC10) ELISA Kit employs a two-site sandwich ELISA to quantitate SIGLEC10 in samples. An antibody specific for SIGLEC10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySIGLEC10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SIGLEC10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SIGLEC10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702878	KTE60662	48 T	$339		Add to Cart
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