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Home / ELISA Kit

Human Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial (ACADSB) ELISA Kit

SKU : BHE13703088

Short/branched chain acyl-CoA dehydrogenase(ACADSB) is a member of the acyl-CoA dehydrogenase family of enzymes that catalyze the dehydrogenation of acyl-CoA derivatives in the metabolism of fatty acids or branch chained amino acids. Substrate specificity is the primary characteristic used to define members of this gene family.
The ACADSB gene product has the greatest activity towards the short branched chain acyl-CoA derivative, (S)-2-methylbutyryl-CoA, but also reacts significantly with other 2-methyl branched chain substrates and with short straight chain acyl-CoAs. The cDNA encodes for a mitochondrial precursor protein which is cleaved upon mitochondrial import and predicted to yield a mature peptide of approximately 43.7-KDa

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Mfr.No.	KTE60872
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial (ACADSB) ELISA Kit employs a two-site sandwich ELISA to quantitate ACADSB.
Alternative Names	ACADSB; 2-MEBCAD; ACAD7; SBCAD; 2-methyl branched chain acyl-CoA dehydrogenase; 2-methylbutyryl-CoA dehydrogenase
Detection Method	Colorimetric
Kit Components	Human Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial microplate Human Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial standard Human Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial (ACADSB) ELISA Kit has high sensitivity and excellent specificity for detection of Human ACADSB. No significant cross-reactivity or interference between Human ACADSB and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Short/branched chain acyl-CoA dehydrogenase(ACADSB) is a member of the acyl-CoA dehydrogenase family of enzymes that catalyze the dehydrogenation of acyl-CoA derivatives in the metabolism of fatty acids or branch chained amino acids. Substrate specificity is the primary characteristic used to define members of this gene family. The ACADSB gene product has the greatest activity towards the short branched chain acyl-CoA derivative, (S)-2-methylbutyryl-CoA, but also reacts significantly with other 2-methyl branched chain substrates and with short straight chain acyl-CoAs. The cDNA encodes for a mitochondrial precursor protein which is cleaved upon mitochondrial import and predicted to yield a mature peptide of approximately 43.7-KDa
Notes	This Human Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial (ACADSB) ELISA Kit employs a two-site sandwich ELISA to quantitate ACADSB in samples. An antibody specific for ACADSB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACADSB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACADSB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACADSB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	36

Applications	ELISA
Applications Notes	This Human Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial (ACADSB) ELISA Kit employs a two-site sandwich ELISA to quantitate ACADSB in samples. An antibody specific for ACADSB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACADSB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACADSB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACADSB bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703088	KTE60872	48 T	$339		Add to Cart
BHE13703088	KTE60872	96 T	$589		Add to Cart
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BHE13703088	KTE60872	96 Tx50	$24569		Add to Cart

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