Human SHMT2(Serine Hydroxymethyltransferase 2, Mitochondrial) ELISA Kit

Mfr.No.

ELK0832

Alternative Names

GLYA; SHMT; mSHMT; NEDCASB; HEL-S-51e

Reactivity

Human

Detection Range

0.16-10 ng/mL

Sample Type

Tissue homogenates, Cell lysates, Other biological fluids, Serum, Plasma

Assay Type

Sandwich

Assay Duration

3.5h

Recovery

Matrices listed below were spiked with certain level of recombinant SHMT2 and the recovery rates were calculated by comparing the measured value to the expected amount of SHMT2 in samples.

Matrix	Recovery range	Average
serum(n=5)	81-103%	87%
EDTA plasma(n=5)	93-105%	99%
Heparin plasma(n=5)	84-93%	89%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SHMT2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	80-101%	87-96%	96-105%	95-103%
EDTA plasma(n=5)	95-103%	87-98%	85-92%	79-96%
Heparin plasma(n=5)	86-96%	85-97%	89-101%	85-97%

Typical Data

Concentration (ng/mL)	OD	Corrected OD
10.00	2.345	2.251
5.00	1.769	1.675
2.50	1.225	1.131
1.25	0.917	0.823
0.63	0.583	0.489
0.32	0.366	0.272
0.16	0.256	0.162
0.00	0.094	0.000

Sensitivity

0.063 ng/mL

Limit Of Detection

10 ng/mL

UniProt ID	P34897
Usage Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SHMT2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SHMT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SHMT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SHMT2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	Enzyme & Kinase;
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE15208442	ELK0832	48T	$320		Add to Cart
BHE15208442	ELK0832	96T	$458		Add to Cart

Human SHMT2(Serine Hydroxymethyltransferase 2, Mitochondrial) ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human SHMT2(Serine Hydroxymethyltransferase 2, Mitochondrial) ELISA Kit

Rate it now..!!