Human SHBG / Sex Hormone Binding Globulin ELISA Kit PicoKine®

Mfr.No.

EK1236

Sensitivity

<0.001 nmol/L

Detection Range

0.031 nmol/L - 2 nmol/L

Reactivity

Human

Sample Volume

Concentration (nmol/L)	0	0.031	0.062	0.125	0.25	0.5	1	2
O.D.	0.075	0.245	0.352	0.524	0.896	1.256	1.802	2.263

Description

Human SHBG / Sex Hormone Binding Globulin ELISA Kit PicoKine® (96 Tests). Quantitate Human SHBG in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 0.001 nmol/L. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Sex hormone-binding globulin;SHBG;Sex steroid-binding protein;SBP;Testis-specific androgen-binding protein;ABP;Testosterone-estradiol-binding globulin;TeBG;Testosterone-estrogen-binding globulin;SHBG;

Molecular Weight

43779 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: L30-H402

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human SHBG

Subcellular Localization

Secreted . In testis, it is synthesized by the Sertoli cells, secreted into the lumen of the seminiferous tubule and transported to the epididymis. .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (nmol/L)	60	301	945	61	312	954
Standard deviation	3	18.66	60.48	3.66	22.46	67.73
CV (%)	5%	6.2%	6.4%	6%	7.2%	7.1%

Protein Function

Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration.

Background

Sex hormone-binding globulin (SHBG) or sex steroid-binding globulin (SSBG) is a glycoprotein that binds to the two sex hormones: androgen and estrogen. The gene for SHBG is located on chromosome 17 on the short arm between the bands 17p12?p13. And this gene encodes a steroid binding protein that was first described as a plasma protein secreted by the liver but is now thought to participate in the regulation of steroid responses. The encoded protein transports androgens and estrogens in the blood, binding each steroid molecule as a dimer formed from identical or nearly identical monomers. Polymorphisms in this gene have been associated with polycystic ovary syndrome and type 2 diabetes mellitus. Alteative splicing results in multiple transcript variants.

Reproducibility

Lots	Lot 1 (nmol/L)	Lot 2 (nmol/L)	Lot 3 (nmol/L)	Lot 4 (nmol/L)	Mean (nmol/L)	Standard Deviation	CV (%)
Sample 1	60	62	64	64	62	1.65	2.6%
Sample 2	301	260	295	294	287	16.1	5.6%
Sample 3	945	910	861	916	908	30.19	3.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	6462
Gene Name	SHBG
UniProt ID	P04278
Protein Name	Sex hormone-binding globulin
Tissue Specificity	Isoform 1 and isoform 2 are present in liver and testis.
Sequence	Contains 2 laminin G-like domains.

Applications	ELISA
Research Area	Cancer,Growth Factors/Hormones,Lipid Metabolism,Metabolism,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001029	EK1236	96 wells/kit	$499		Add to Cart

Human SHBG / Sex Hormone Binding Globulin ELISA Kit PicoKine®

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