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Home / ELISA Kit

Human SH3 domain-binding glutamic acid-rich-like protein (SH3BGRL) ELISA Kit

SKU : BHE13702886

Proline-rich peptide sequences have been shown to play important roles in protein-protein interactions that occur in signal transduction pathways. Specific proline-rich consensus sequences that bind SH3 domains have been identified.
The predicted 114-amino acid SH3BGRL protein has a potential cell attachment sequence. A proline-rich sequence containing an SH3-binding motif is present in the middle of the protein. The SH3BGRL protein shares 60% identity with the proline-rich central region of SH3BGR. Northern blot analysis detected a single SH3BGRL transcript of approximately 1.9 kb that was present in all tissues examined.Mouse cDNA encoding a deduced protein that is 95% identical to human SH3BGRL.

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Mfr.No.	KTE60670
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human SH3 domain-binding glutamic acid-rich-like protein (SH3BGRL) ELISA Kit employs a two-site sandwich ELISA to quantitate SH3BGRL.
Alternative Names	SH3BGRL; MGC117402; SH3BGR; SH3-binding domain glutamic acid-rich protein like
Detection Method	Colorimetric
Kit Components	Human SH3 domain-binding glutamic acid-rich-like protein microplate Human SH3 domain-binding glutamic acid-rich-like protein standard Human SH3 domain-binding glutamic acid-rich-like protein detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human SH3 domain-binding glutamic acid-rich-like protein (SH3BGRL) ELISA Kit has high sensitivity and excellent specificity for detection of Human SH3BGRL. No significant cross-reactivity or interference between Human SH3BGRL and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Proline-rich peptide sequences have been shown to play important roles in protein-protein interactions that occur in signal transduction pathways. Specific proline-rich consensus sequences that bind SH3 domains have been identified. The predicted 114-amino acid SH3BGRL protein has a potential cell attachment sequence. A proline-rich sequence containing an SH3-binding motif is present in the middle of the protein. The SH3BGRL protein shares 60% identity with the proline-rich central region of SH3BGR. Northern blot analysis detected a single SH3BGRL transcript of approximately 1.9 kb that was present in all tissues examined.Mouse cDNA encoding a deduced protein that is 95% identical to human SH3BGRL.
Notes	This Human SH3 domain-binding glutamic acid-rich-like protein (SH3BGRL) ELISA Kit employs a two-site sandwich ELISA to quantitate SH3BGRL in samples. An antibody specific for SH3BGRL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySH3BGRL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SH3BGRL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SH3BGRL bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	83699

Applications	ELISA
Applications Notes	This Human SH3 domain-binding glutamic acid-rich-like protein (SH3BGRL) ELISA Kit employs a two-site sandwich ELISA to quantitate SH3BGRL in samples. An antibody specific for SH3BGRL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySH3BGRL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SH3BGRL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SH3BGRL bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702886	KTE60670	48 T	$339		Add to Cart
BHE13702886	KTE60670	96 T	$589		Add to Cart
BHE13702886	KTE60670	96 Tx5	$2629		Add to Cart
BHE13702886	KTE60670	96 Tx50	$24569		Add to Cart

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