Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of SGPP2 and the recovery rates were calculated by comparing the measured value to the expected amount of SGPP2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-104
95
EDTA plasma(n=5)
96-99
97
heparin plasma(n=5)
85-101
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SGPP2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-101%
87-101%
86-104%
EDTA plasma(n=5)
88-97%
88-97%
87-99%
heparin plasma(n=5)
88-100%
86-99%
92-100%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
Q8IWX5
Applications
ELISA
Applications Notes
SGPP2 ELISA Kit allows for the in vitro quantitative determination of SGPP2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human SGPP2 (Sphingosine-1-phosphate phosphatase 2) ELISA Kit