Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of SFN and the recovery rates were calculated by comparing the measured value to the expected amount of SFN in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-105
98
EDTA plasma(n=5)
90-104
96
heparin plasma(n=5)
85-100
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SFN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
SFN/14-3-3 protein sigma/Epithelial cell marker protein 1/Stratifin/HME1/YWHAS
Notes
For Research Use Only
UniProt ID
P31947
Antigen
SFN
Applications
ELISA
Applications Notes
SFN ELISA Kit allows for the in vitro quantitative determination of SFN concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human SFN (14-3-3 protein sigma) ELISA Kit