Human SEZ6L ELISA Kit PicoKine®

Mfr.No.

EK1862

Sensitivity

<0.1 ng/ml

Detection Range

0.312 ng/ml - 20 ng/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	312.5	625	1250	2500	5000	10000	20000
O.D.	0.041	0.141	0.193	0.341	0.598	0.852	1.531	2.056

Description

Human SEZ6L ELISA Kit PicoKine® (96 Tests). Quantitate Human SEZ6L in cell culture supeatants, serum and plasma (heparin). Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Seizure 6-like protein; SEZ6L; KIAA0927; UNQ2542/PRO6094

Immunogen

Expression system for standard: NS0; Immunogen sequence: L29-I1024

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human SEZ6L

Subcellular Localization

Endoplasmic reticulum membrane, Single-pass type I membrane protein

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	489	1734	10155	544	2012	11046
Standard deviation	31.30	97.10	710.85	23.39	114.68	839.50
CV (%)	6.4%	5.6%	7.0%	4.3%	5.7%	7.6%

Protein Function

May contribute to specialized endoplasmic reticulum functions in neurons.

Background

Seizure 6-like protein is a protein that in humans is encoded by the SEZ6L gene. This gene is mapped to 22q12.1.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	489	478	517	524	502	19.06	3.7%
Sample 2	1734	1756	1906	1797	1798	66.19	3.6%
Sample 3	10155	8845	9766	8917	9420	557.57	5.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	23544
Gene Name	SEZ6L
UniProt ID	Q9BYH1
Tissue Specificity	Widely expressed, including adult and fetal brains and lungs. Not expressed in all lung cancer cell lines.

Applications	ELISA
Research Area	Cancer,Intracellular Signaling,Neuroscience,Neurotransmission,Oncoproteins/Suppressors,Secretory Vesicles,Tumor Suppressors

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001421	EK1862	96 wells/kit	$750		Add to Cart

Human SEZ6L ELISA Kit PicoKine®

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