Matrices listed below were spiked with certain level of SERPINA10 and the recovery rates were calculated by comparing the measured value to the expected amount of SERPINA10 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-100
94
EDTA plasma(n=5)
82-94
88
heparin plasma(n=5)
90-100
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SERPINA10 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-103%
86-103%
82-88%
EDTA plasma(n=5)
84-99%
83-101%
81-96%
heparin plasma(n=5)
82-100%
80-93%
82-92%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
Q9UK55
Applications
ELISA
Applications Notes
SERPINA10 ELISA Kit allows for the in vitro quantitative determination of SERPINA10 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human SERPINA10(Protein Z-dependent protease inhibitor) ELISA Kit