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Home / ELISA Kit

Human Serpin H1 (SERPINH1) ELISA Kit

SKU : BHE13702905

Heat shock protein 47 is a member of the serpin superfamily of serine proteinase inhibitors. Its expression is induced by heat shock. The protein localizes to the endoplasmic reticulum lumen and binds collagen; thus it is thought to be a molecular chaperone involved in the maturation of collagen molecules. Autoantibodies to this protein have been found in patients with rheumatoid arthritis. Colligins are characterized by an amino acid structure that includes an N-terminal hydrophobic signal sequence and 2 putative N-linked oligosaccharide attachment sites (Clarke et al., 1991). Colligins also have a C-terminal RDEL sequence that acts as an endoplasmic reticulum (ER) retention sequence. Other features permit the colligin-binding protein of ER to be classified as a serpin (serine-arginine protease inhibitor).

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Mfr.No.	KTE60689
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Serpin H1 (SERPINH1) ELISA Kit employs a two-site sandwich ELISA to quantitate SERPINH1.
Alternative Names	SERPINH1; AsTP3; CBP1; CBP2; HSP47; PIG14; PPROM; RA-A47; SERPINH2; gp46; arsenic-transactivated protein 3; colligin-1; colligin-2; proliferation-inducing gene 14; rheumatoid arthritis antigen A-47; serine (or cys
Detection Method	Colorimetric
Kit Components	Human Serpin H1 microplate Human Serpin H1 standard Human Serpin H1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Serpin H1 (SERPINH1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SERPINH1. No significant cross-reactivity or interference between Human SERPINH1 and analogues was observed.
Calibration Range	Please inquire
Sample Type	Serum, Cell culture supeatants, Plasma, Other biological fluids
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Heat shock protein 47 is a member of the serpin superfamily of serine proteinase inhibitors. Its expression is induced by heat shock. The protein localizes to the endoplasmic reticulum lumen and binds collagen; thus it is thought to be a molecular chaperone involved in the maturation of collagen molecules. Autoantibodies to this protein have been found in patients with rheumatoid arthritis. Colligins are characterized by an amino acid structure that includes an N-terminal hydrophobic signal sequence and 2 putative N-linked oligosaccharide attachment sites (Clarke et al., 1991). Colligins also have a C-terminal RDEL sequence that acts as an endoplasmic reticulum (ER) retention sequence. Other features permit the colligin-binding protein of ER to be classified as a serpin (serine-arginine protease inhibitor).
Notes	This Human Serpin H1 (SERPINH1) ELISA Kit employs a two-site sandwich ELISA to quantitate SERPINH1 in samples. An antibody specific for SERPINH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySERPINH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	510850

Applications	ELISA
Applications Notes	This Human Serpin H1 (SERPINH1) ELISA Kit employs a two-site sandwich ELISA to quantitate SERPINH1 in samples. An antibody specific for SERPINH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySERPINH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702905	KTE60689	48 T	$339		Add to Cart
BHE13702905	KTE60689	96 T	$589		Add to Cart
BHE13702905	KTE60689	96 Tx5	$2629		Add to Cart
BHE13702905	KTE60689	96 Tx50	$24569		Add to Cart

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