Human Serpin F2/alpha 2-Antiplasmin/SERPINF2 ELISA Kit PicoKine®

Mfr.No.

EK1712

Sensitivity

<10 pg/ml

Detection Range

1.56 ng/ml - 100 ng/ml

Reactivity

Human

Sample Volume

Concentration (ng/ml)	0	1.56	3.13	6.25	12.5	25	50	100
O.D.	0.074	0.253	0.335	0.536	0.815	1.219	1.671	1.915

Description

Human Serpin F2/alpha 2-Antiplasmin/SERPINF2 ELISA Kit PicoKine® (96 Tests). Quantitate Human SERPINF2 in cell culture supeatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Fibroblast growth factor-binding protein 1; FGF-BP; FGF-BP1; FGF-binding protein 1; FGFBP-1; Growth factor-binding protein 1; Fgfbp1

Immunogen

Expression system for standard: NS0; Immunogen sequence: N40-K491

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human SerpinF2

Subcellular Localization

Secreted, extracellular space

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	1883	10402	40754	1930	12110	43978
Standard deviation	114.86	488.89	2282.2	123.52	884.03	3430.28
CV (%)	6.1%	4.7%	5.6%	6.4%	7.3%	7.8%

Protein Function

Acts as a carrier protein that release fibroblast- binding factors (FGFs) from the extracellular matrix (EM) storage and thus enhance the mitogenic activity of FGFs. Enhances FGF2 signaling during tissue repair, angiogenesis and in tumor growth (By similarity). .

Background

Human SerpinF2 encodes a member of the serpin family of serine protease inhibitors. The protein is a major inhibitor of plasmin, which degrades fibrin and various other proteins. Consequently, the proper function of this gene has a major role in regulating the blood clotting pathway. Mutations in this gene result in alpha-2-plasmin inhibitor deficiency, which is characterized by severe hemorrhagic diathesis. Multiple transcript variants encoding different isoforms have been found for this gene.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	1883	2175	2014	2130	2050	113.14	5.5%
Sample . 2	10402	11339	11464	10592	10949	459.34	4.1%%
Sample 3	40754	39524	41038	45998	41828	2473.60	5.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	5345
Gene Name	SERPINF2
UniProt ID	P08697
Tissue Specificity	Expressed in gut, eye, thymus, skin, lung, tongue, Purkinje cells and cerebral chorioid plexus (at protein level).

Applications	ELISA
Research Area	Angiogenesis,Cardiovascular,Cell Biology,Neurogenesis,Neurology Process,Neuroscience,Protease Inhibitors,Proteolysis/Ubiquitin,Sensory System,Visual System

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001372	EK1712	96 wells/kit	$499		Add to Cart

Human Serpin F2/alpha 2-Antiplasmin/SERPINF2 ELISA Kit PicoKine®

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