Human Serpin B5(SERPINB5) ELISA kit

Mfr.No.

CSB-EL021073HU

Alternative Names

serpin peptidase inhibitor, clade B (ovalbumin), member 5, PI5, maspin, protease inhibitor 5 (maspin), serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5

Reactivity

Human

Detection Range

0.156 ng/mL-10 ng/mL

Sample Type

Tissue homogenates, Cell lysates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human IGHA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	83-101
EDTA plasma (n=4)	95	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IGHA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:5000	Average %	92
	Range %	85-103
1:10000	Average %	91
	Range %	85-99
1:20000	Average %	97
	Range %	90-108
1:40000	Average %	92
	Range %	83-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
2000	2.234	2.285	2.260	2.171
500	2.112	2.165	2.139	2.050
125	1.886	1.897	1.892	1.803
31.25	1.265	1.245	1.255	1.166
7.81	0.608	0.587	0.598	0.509
1.95	0.290	0.286	0.288	0.199
0.49	0.136	0.139	0.138	0.049
0	0.086	0.091	0.089	?

Species

Homo sapiens

Sensitivity

0.039 ng/mL

Limit Of Detection

450 nm

UniProt ID	P36952
Antigen	serpin peptidase inhibitor, clade B (ovalbumin), member 5

Applications

ELISA

Research Area

SERPINB5

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504306	CSB-EL021073HU	96T	$695	Add to Cart
BHE10504306	CSB-EL021073HU	5x96T	$3336	Add to Cart
BHE10504306	CSB-EL021073HU	10x96T	$6602.50	Add to Cart

Human Serpin B5(SERPINB5) ELISA kit

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