Human Serpin A1/alpha 1-Antitrypsin ELISA Kit PicoKine®

Mfr.No.

EK1634

Sensitivity

<10 pg/ml

Detection Range

1.56 ng/ml - 100 ng/ml

Reactivity

Human

Sample Volume

Concentration (ng/ml)	0	1.56	3.12	6.25	12.5	25	50	100
O.D.	0.063	0.112	0.217	0.403	0.679	1.050	1.612	2.028

Description

Human Serpin A1/alpha 1-Antitrypsin ELISA Kit PicoKine® (96 Tests). Quantitate Human SERPINA1 in cell culture supeatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Alpha-1-antitrypsin; Alpha-1 protease inhibitor; Alpha-1-antiproteinase; Serpin A1; Short peptide from AAT; SPAAT; SERPINA1; AAT; PI; PRO0684; PRO2209

Immunogen

Expression system for standard: NS0; Immunogen sequence: E25-K418

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Serpin A1

Subcellular Localization

Secreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum.

Sample Type

Cell culture supeatants, Serum, Plasma, Urine

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	2451	15117	38332	2323	15142	40979
Standard deviation	144.6	1179.12	2529.91	157.96	1423.34	3114.4
CV (%)	6.6%%	7.8%	6.6%	6.8%	9.4%	7.6%

Protein Function

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.

Background

Alpha-1-antitrypsin?or??1-antitrypsin? (A1AT,?A1A, or?AAT) is a?protein?belonging to the?serpin?superfamily. It is encoded in humans by the?SERPINA1?gene.?The?gene?is located on the long arm of the fourteenth?chromosome? (14q32.1).The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene.Like all?serine protease inhibitors, A1AT has a characteristic?secondary structure?of?beta sheets?and?alpha helices.?Mutations?in these areas can lead to non-functional proteins that can?polymerise?and accumulate in the?liver (infantile hepatic cirrhosis).

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	2451	2297	2432	2102	2320	139.42	6%
Sample 2	15117	15111	14581	14540	14837	277.13	1.8%
Sample 3	38332	38692	42108	38880	39503	1516.83	3.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	5265
Gene Name	SERPINA1
UniProt ID	P01009
Tissue Specificity	Ubiquitous. Expressed in leukocytes and plasma.

Applications	ELISA
Research Area	Intracellular,Kinases,Metabolism,Metabolism Processes,Pathways and Processes,Protease Inhibitors,Proteolysis/Ubiquitin,Response To Hypoxia,Serum Proteins

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001348	EK1634	96 wells/kit	$499		Add to Cart

Human Serpin A1/alpha 1-Antitrypsin ELISA Kit PicoKine®

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