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Home / ELISA Kit

Human Serotonin N-acetyltransferase (AANAT) ELISA Kit

SKU : BHE13703062

Serotonin N-acetyl transferase is an enzyme involved in the conversion of serotonin to melatonin in pinealocytes. It is an acetyl-CoA dependent enzyme of the GCN5-related family of N-acetyltransferases (GNATs). Arylalkylamine N-acetyltransferase is the penultimate enzyme in melatonin synthesis and controls the night/day rhythm in melatonin production in the vertebrate pineal gland. Melatonin is essential for seasonal reproduction, modulates the function of the circadian clock in the suprachiasmatic nucleus, and influences activity and sleep. This enzyme is rapidly inactivated when animals are exposed to light at night. This protein is 80% identical to sheep and rat AA-NAT. Arylalkylamine N-acetyltransferase may contribute a multifactorial genetic diseases such as altered behavior in sleep/wake cycle.

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Mfr.No.	KTE60846
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Serotonin N-acetyltransferase (AANAT) ELISA Kit employs a two-site sandwich ELISA to quantitate AANAT.
Alternative Names	AANAT; SNAT; serotonin acetylase
Detection Method	Colorimetric
Kit Components	Human Serotonin N-acetyltransferase microplate Human Serotonin N-acetyltransferase standard Human Serotonin N-acetyltransferase detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Serotonin N-acetyltransferase (AANAT) ELISA Kit has high sensitivity and excellent specificity for detection of Human AANAT. No significant cross-reactivity or interference between Human AANAT and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Serotonin N-acetyl transferase is an enzyme involved in the conversion of serotonin to melatonin in pinealocytes. It is an acetyl-CoA dependent enzyme of the GCN5-related family of N-acetyltransferases (GNATs). Arylalkylamine N-acetyltransferase is the penultimate enzyme in melatonin synthesis and controls the night/day rhythm in melatonin production in the vertebrate pineal gland. Melatonin is essential for seasonal reproduction, modulates the function of the circadian clock in the suprachiasmatic nucleus, and influences activity and sleep. This enzyme is rapidly inactivated when animals are exposed to light at night. This protein is 80% identical to sheep and rat AA-NAT. Arylalkylamine N-acetyltransferase may contribute a multifactorial genetic diseases such as altered behavior in sleep/wake cycle.
Notes	This Human Serotonin N-acetyltransferase (AANAT) ELISA Kit employs a two-site sandwich ELISA to quantitate AANAT in samples. An antibody specific for AANAT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAANAT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AANAT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AANAT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	15

Applications	ELISA
Applications Notes	This Human Serotonin N-acetyltransferase (AANAT) ELISA Kit employs a two-site sandwich ELISA to quantitate AANAT in samples. An antibody specific for AANAT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAANAT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AANAT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AANAT bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703062	KTE60846	48 T	$339		Add to Cart
BHE13703062	KTE60846	96 T	$589		Add to Cart
BHE13703062	KTE60846	96 Tx5	$2629		Add to Cart
BHE13703062	KTE60846	96 Tx50	$24569		Add to Cart

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