Human Serine Threonine-protein Phosphatase 2A 65 kDa Regulatory Subunit A Alpha Isoform

Mfr.No.

E6152Hu

Sensitivity

9.02ng/L

Detection Range

20-3500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Medium tumor antigen-associated 61 kDa protein;MRD36;PP2A subunit A isoform PR65-alpha;PP2A subunit A isoform R1-alpha;PP2AA;PP2AAALPHA;PP2A-Aalpha;PPP2R1A;PR65A;Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform

Molecular Weight

65309

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E6152Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	549.84	22.52	4.1
Intra-Assay	2	18	156.23	7.24	4.63
Intra-Assay	3	18	339.65	18.08	5.32

Background

The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Upon interaction with GNA12 promotes dephosphorylation of microtubule associated protein TAU/MAPT (PubMed:15525651). Required for proper chromosome segregation and for centromeric localization of SGO1 in mitosis (PubMed:16580887).

Protocol

These standard curves of E6152Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2000ng/L	2.145	2.092	2.043
	2.039
1000ng/L	1.761	1.854	1.805
	1.947
500ng/L	1.299	1.237	1.188
	1.176
250ng/L	0.701	0.724	0.675
	0.746
125ng/L	0.441	0.399	0.35
	0.356
0ng/L	0.054	0.049	0
	0.044

Gene ID	5518
UniProt ID	P30153

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107514	E6152Hu	48T	$320		Add to Cart
BHE12107514	E6152Hu	96T	$458		Add to Cart

Human Serine Threonine-protein Phosphatase 2A 65 kDa Regulatory Subunit A Alpha Isoform - PPP2R1A ELISA Kit

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