Human Serine Threonine-protein Kinase Wnk2

Mfr.No.

E6149Hu

Sensitivity

7.55ng/L

Detection Range

15-3000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Antigen NY-CO-43;NY-CO-43;P/OKcl.13;PRKWNK2;Protein kinase lysine-deficient 2;Protein kinase with no lysine 2;SDCCAG43;Serine/threonine-protein kinase WNK2;Serologically defined colon cancer antigen 43;WNK2

Molecular Weight

242676

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E6149Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1178.74	45.46	3.86
Intra-Assay	2	18	731.52	42.15	5.76
Intra-Assay	3	18	195.65	12.22	6.25

Background

Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SLC12A2, SCNN1A, SCNN1B, SCNN1D and SGK1 and inhibits SLC12A5. Negatively regulates the EGF-induced activation of the ERK/MAPK-pathway and the downstream cell cycle progression. Affects MAPK3/MAPK1 activity by modulating the activity of MAP2K1 and this modulation depends on phosphorylation of MAP2K1 by PAK1. WNK2 acts by interfering with the activity of PAK1 by controlling the balance of the activity of upstream regulators of PAK1 activity, RHOA and RAC1, which display reciprocal activity.

Protocol

These standard curves of E6149Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.309	2.268	2.21
	2.227
800ng/L	1.299	1.284	1.227
	1.269
400ng/L	0.761	0.737	0.68
	0.713
200ng/L	0.493	0.493	0.436
	0.493
100ng/L	0.244	0.212	0.155
	0.18
0ng/L	0.054	0.057	0
	0.06

Gene ID	65268
UniProt ID	Q9Y3S1

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107511	E6149Hu	48T	$320		Add to Cart
BHE12107511	E6149Hu	96T	$458		Add to Cart

Human Serine Threonine-protein Kinase Wnk2 - WNK2 ELISA Kit

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