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Home / ELISA Kit

Human Serine/threonine-protein kinase RIO1 (RIOK1) ELISA Kit

SKU : BHE13703011

RIOK1 includes two alternatively spliced transcript variants, which encode different isoforms. The function of this gene has not been determined. RioK1, a new interactor of protein arginine methyltransferase 5 (PRMT5), competes with pICln for binding and modulates PRMT5 complex composition and substrate specificity. RioK1 and pICln bind to PRMT5 in a mutually exclusive fashion. This results in a PRMT5-WD45/MEP50 core structure that either associates with pICln or RioK1 in distinct complexes. RioK1 functions in analogy to pICln as an adapter protein by recruiting the RNA-binding protein nucleolin to the PRMT5 complex for its symmetrical methylation. The exclusive interaction of PRMT5 with either pICln or RioK1 thus provides the first mechanistic insight into how a methyltransferase can distinguish between its substrate proteins.

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Mfr.No.	KTE60795
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Serine/threonine-protein kinase RIO1 (RIOK1) ELISA Kit employs a two-site sandwich ELISA to quantitate RIOK1.
Alternative Names	RIOK1; AD034; FLJ30006; MGC12903; bA288G3.1; RIO kinase 1; prematurely truncated due to insertion of intronic sequence
Detection Method	Colorimetric
Kit Components	Human Serine/threonine-protein kinase RIO1 microplate Human Serine/threonine-protein kinase RIO1 standard Human Serine/threonine-protein kinase RIO1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Serine/threonine-protein kinase RIO1 (RIOK1) ELISA Kit has high sensitivity and excellent specificity for detection of Human RIOK1. No significant cross-reactivity or interference between Human RIOK1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	RIOK1 includes two alternatively spliced transcript variants, which encode different isoforms. The function of this gene has not been determined. RioK1, a new interactor of protein arginine methyltransferase 5 (PRMT5), competes with pICln for binding and modulates PRMT5 complex composition and substrate specificity. RioK1 and pICln bind to PRMT5 in a mutually exclusive fashion. This results in a PRMT5-WD45/MEP50 core structure that either associates with pICln or RioK1 in distinct complexes. RioK1 functions in analogy to pICln as an adapter protein by recruiting the RNA-binding protein nucleolin to the PRMT5 complex for its symmetrical methylation. The exclusive interaction of PRMT5 with either pICln or RioK1 thus provides the first mechanistic insight into how a methyltransferase can distinguish between its substrate proteins.
Notes	This Human Serine/threonine-protein kinase RIO1 (RIOK1) ELISA Kit employs a two-site sandwich ELISA to quantitate RIOK1 in samples. An antibody specific for RIOK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRIOK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RIOK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RIOK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	83732

Applications	ELISA
Applications Notes	This Human Serine/threonine-protein kinase RIO1 (RIOK1) ELISA Kit employs a two-site sandwich ELISA to quantitate RIOK1 in samples. An antibody specific for RIOK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRIOK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RIOK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RIOK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703011	KTE60795	48 T	$339		Add to Cart
BHE13703011	KTE60795	96 T	$589		Add to Cart
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BHE13703011	KTE60795	96 Tx50	$24569		Add to Cart

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