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Home / ELISA Kit

Human Serine/threonine-protein kinase PLK3 (PLK3) ELISA Kit

SKU : BHE13703422

CNK is a member of the 'polo' family of serine/threonine kinases. Members of this family have been implicated in cell division and are important in regulating the onset of mitosis and M-phase progression.The predicted 607-amino acid protein has an N-terminal catalytic domain, including an ATP-binding site, a central putative nuclear targeting signal, and a presumed C-terminal regulatory domain. The CNK protein shows homology with several other members of the 'polo' family; it is 91% identical to mouse Fnk, 50% identical to human PLK, 48% identical to Drosophila polo, and 38% identical to S. cerevisiae Cdc5. Northern blot analysis detected expression of a 2.5-kb CNK transcript in a limited number of human tissues, with placenta containing a moderate level and ovary, lung, and peripheral blood leukocytes containing low levels.

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Mfr.No.	KTE61206
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Serine/threonine-protein kinase PLK3 (PLK3) ELISA Kit employs a two-site sandwich ELISA to quantitate PLK3.
Alternative Names	PLK3; RP11-269F19.6; CNK; FNK; PRK; FGF-inducible kinase; cytokine-inducible kinase; polo-like kinase 3; proliferation-related kinase
Detection Method	Colorimetric
Kit Components	Human Serine/threonine-protein kinase PLK3 microplate Human Serine/threonine-protein kinase PLK3 standard Human Serine/threonine-protein kinase PLK3 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Serine/threonine-protein kinase PLK3 (PLK3) ELISA Kit has high sensitivity and excellent specificity for detection of Human PLK3. No significant cross-reactivity or interference between Human PLK3 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	CNK is a member of the 'polo' family of serine/threonine kinases. Members of this family have been implicated in cell division and are important in regulating the onset of mitosis and M-phase progression.The predicted 607-amino acid protein has an N-terminal catalytic domain, including an ATP-binding site, a central putative nuclear targeting signal, and a presumed C-terminal regulatory domain. The CNK protein shows homology with several other members of the 'polo' family; it is 91% identical to mouse Fnk, 50% identical to human PLK, 48% identical to Drosophila polo, and 38% identical to S. cerevisiae Cdc5. Northern blot analysis detected expression of a 2.5-kb CNK transcript in a limited number of human tissues, with placenta containing a moderate level and ovary, lung, and peripheral blood leukocytes containing low levels.
Notes	This Human Serine/threonine-protein kinase PLK3 (PLK3) ELISA Kit employs a two-site sandwich ELISA to quantitate PLK3 in samples. An antibody specific for PLK3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLK3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLK3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLK3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	1263

Applications	ELISA
Applications Notes	This Human Serine/threonine-protein kinase PLK3 (PLK3) ELISA Kit employs a two-site sandwich ELISA to quantitate PLK3 in samples. An antibody specific for PLK3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLK3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLK3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLK3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703422	KTE61206	48 T	$339		Add to Cart
BHE13703422	KTE61206	96 T	$589		Add to Cart
BHE13703422	KTE61206	96 Tx5	$2629		Add to Cart
BHE13703422	KTE61206	96 Tx50	$24569		Add to Cart

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