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Home / ELISA Kit

Human Serine/threonine-protein kinase PAK 4 (PAK4) ELISA Kit

SKU : BHE13703530

PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities.
PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.Highest expression in prostate, testis and colon.

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Mfr.No.	KTE61314
Description	This Human Serine/threonine-protein kinase PAK 4 (PAK4) ELISA Kit employs a two-site sandwich ELISA to quantitate PAK4.
Alternative Names	PAK4; p21(CDKN1A)-activated kinase 4; p21-activated kinase 4; protein kinase related to S. cerevisiae STE20; effector for Cdc42Hs
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Serine/threonine-protein kinase PAK 4 microplate Human Serine/threonine-protein kinase PAK 4 standard Human Serine/threonine-protein kinase PAK 4 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Serine/threonine-protein kinase PAK 4 (PAK4) ELISA Kit has high sensitivity and excellent specificity for detection of Human PAK4. No significant cross-reactivity or interference between Human PAK4 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.Highest expression in prostate, testis and colon.
Notes	This Human Serine/threonine-protein kinase PAK 4 (PAK4) ELISA Kit employs a two-site sandwich ELISA to quantitate PAK4 in samples. An antibody specific for PAK4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPAK4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PAK4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PAK4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	10298
Antigen	PAK4
Tag	PAK4
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Serine/threonine-protein kinase PAK 4 (PAK4) ELISA Kit employs a two-site sandwich ELISA to quantitate PAK4 in samples. An antibody specific for PAK4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPAK4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PAK4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PAK4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703530	KTE61314	48 T	$339		Add to Cart
BHE13703530	KTE61314	96 T	$589		Add to Cart
BHE13703530	KTE61314	96 Tx5	$2629		Add to Cart
BHE13703530	KTE61314	96 Tx50	$24569		Add to Cart

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