Human Serine protease inhibitor Kazal-type 5(SPINK5) ELISA kit

Human Serine protease inhibitor Kazal-type 5(SPINK5) ELISA kit

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Mfr.No.

CSB-EL022578HU

Sensitivity

15.6 pg/mL

Detection Range

62.5 pg/mL-4000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

serine peptidase inhibitor, Kazal type 5, DKFZp686K19184, FLJ21544, FLJ97536, FLJ97596, FLJ99794, LEKTI, LETKI, NETS, NS, VAKTI, OTTHUMP00000165952, lymphoepithelial Kazal-type-related inhibitor, serine protease inhibitor, Kazal type 5

Recovery

The recovery of rat IgE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-98
EDTA plasma (n=4)	95	90-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat IgE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	85
	Range %	84-93
1:2	Average %	96
	Range %	91-103
1:4	Average %	84
	Range %	81-89
1:8	Average %	93
	Range %	89-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
200	2.055	2.086	2.071	1.926
100	1.520	1.649	1.585	1.440
50	1.101	1.156	1.129	0.984
25	0.738	0.750	0.744	0.599
12.5	0.417	0.445	0.431	0.286
6.25	0.341	0.337	0.339	0.194
3.12	0.201	0.213	0.207	0.062
0	0.139	0.150	0.145	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q9NQ38

Applications	ELISA
Research Area	SPINK5

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504280	CSB-EL022578HU	96T	$695	Add to Cart
BHE10504280	CSB-EL022578HU	5x96T	$3336	Add to Cart
BHE10504280	CSB-EL022578HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.