Human Serine protease HTRA1(HTRA1) ELISA kit

Mfr.No.

CSB-EL010901HU

Alternative Names

HtrA serine peptidase 1, ARMD7, HtrA, L56, ORF480, PRSS11, IGFBP5-protease, protease, serine, 11 (IGF binding)

Reactivity

Human

Detection Range

1.56 ng/mL-100 ng/mL

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat LTD4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-101
EDTA plasma (n=4)	92	87-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat LTD4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-97
1:2	Average %	103
	Range %	99-108
1:4	Average %	91
	Range %	85-97
1:8	Average %	94
	Range %	91-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

0.39 ng/mL

Limit Of Detection

450 nm

UniProt ID	Q92743
Antigen	HtrA serine peptidase 1

Applications

ELISA

Research Area

HTRA1

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
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