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Home / ELISA Kit

Human Serine palmitoyltransferase 3 (SPTLC3) ELISA Kit

SKU : BHE13702569

SPTLC3 orthologs were found in rat, mouse, and dog. Quantitative real-time PCR of human tissues showed highest expression in placenta, followed by skin, adrenal gland, testis, uterus, salivary gland, prostate, and kidney, lower expression in all other tissues, and no expression in peripheral blood cells and bone marrow. SPTLC3 and SPTLC2 showed different expression patterns, possibly reflecting tissue-specific requirements of sphingolipid synthesis. Western blot analysis revealed SPTLC3 expression in human trophoblast cell lines. Overexpression of SPTLC3 in HEK293 cells, which have little endogenous SPTLC3, resulted in increased SPT activity by 2- to 3-fold. Expression of an SPTLC3-specific siRNA in HepG2 cells and human trophoblast cells significantly decreased SPT activity, indicating that SPTLC3 plays a role in SPT activity.

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Mfr.No.	KTE60352
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Serine palmitoyltransferase 3 (SPTLC3) ELISA Kit employs a two-site sandwich ELISA to quantitate SPTLC3.
Alternative Names	SPTLC3; RP5-1077I2.1; C20orf38; FLJ11112; FLJ90790; LCB3; SPT3; SPTLC2L; dJ718P11; dJ718P11.1; long chain base biosynthesis protein 3; serine palmitoyltransferase; long chain base subunit 2-like (aminotransf
Detection Method	Colorimetric
Kit Components	Human Serine palmitoyltransferase 3 microplate Human Serine palmitoyltransferase 3 standard Human Serine palmitoyltransferase 3 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Serine palmitoyltransferase 3 (SPTLC3) ELISA Kit has high sensitivity and excellent specificity for detection of Human SPTLC3. No significant cross-reactivity or interference between Human SPTLC3 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	SPTLC3 orthologs were found in rat, mouse, and dog. Quantitative real-time PCR of human tissues showed highest expression in placenta, followed by skin, adrenal gland, testis, uterus, salivary gland, prostate, and kidney, lower expression in all other tissues, and no expression in peripheral blood cells and bone marrow. SPTLC3 and SPTLC2 showed different expression patterns, possibly reflecting tissue-specific requirements of sphingolipid synthesis. Western blot analysis revealed SPTLC3 expression in human trophoblast cell lines. Overexpression of SPTLC3 in HEK293 cells, which have little endogenous SPTLC3, resulted in increased SPT activity by 2- to 3-fold. Expression of an SPTLC3-specific siRNA in HepG2 cells and human trophoblast cells significantly decreased SPT activity, indicating that SPTLC3 plays a role in SPT activity.
Notes	This Human Serine palmitoyltransferase 3 (SPTLC3) ELISA Kit employs a two-site sandwich ELISA to quantitate SPTLC3 in samples. An antibody specific for SPTLC3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPTLC3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPTLC3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPTLC3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	55304

Applications	ELISA
Applications Notes	This Human Serine palmitoyltransferase 3 (SPTLC3) ELISA Kit employs a two-site sandwich ELISA to quantitate SPTLC3 in samples. An antibody specific for SPTLC3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPTLC3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPTLC3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPTLC3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702569	KTE60352	48 T	$339		Add to Cart
BHE13702569	KTE60352	96 T	$589		Add to Cart
BHE13702569	KTE60352	96 Tx5	$2629		Add to Cart
BHE13702569	KTE60352	96 Tx50	$24569		Add to Cart

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