Matrices listed below were spiked with certain level of SEPTIN7 and the recovery rates were calculated by comparing the measured value to the expected amount of SEPTIN7 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-100
97
EDTA plasma(n=5)
90-104
96
heparin plasma(n=5)
88-102
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SEPTIN7 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-97%
86-104%
91-104%
EDTA plasma(n=5)
84-99%
82-96%
82-98%
heparin plasma(n=5)
87-98%
91-96%
86-99%
Sensitivity
< 0.188ng/ml
Notes
For Research Use Only
UniProt ID
Q16181
Antigen
SEPTIN7
Applications
ELISA
Applications Notes
SEPTIN7 ELISA Kit allows for the in vitro quantitative determination of SEPTIN7 concentrations in serum, plasma, tissue homogenates and other biological fluids.