Human Septin-7 - SEPT7 ELISA Kit

Mfr.No.

E3429Hu

Sensitivity

0.02ng/ml

Detection Range

0.05-20ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Human Septin-7 (also known as SEPT7) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CDC10 protein homolog;SEPTIN 7;SEPTIN7;Septin-7

Molecular Weight

50680

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E3429Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	3.4	0.15	4.4
Intra-Assay	2	18	4.9	0.2	4.2
Intra-Assay	3	18	9.8	0.32	3.3

Background

Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Required for normal progress through mitosis. Involved in cytokinesis. Required for normal association of CENPE with the kinetochore. Plays a role in ciliogenesis and collective cell movements. Forms a filamentous structure with SEPTIN12, SEPTIN6, SEPTIN2 and probably SEPTIN4 at the sperm annulus which is required for the structural integrity and motility of the sperm tail during postmeiotic differentiation (PubMed: 25588830).

Protocol

These standard curves of E3429Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	1.675	1.692	1.636
	1.71
6ng/ml	0.956	0.972	0.916
	0.988
3ng/ml	0.513	0.498	0.442
	0.484
1.5ng/ml	0.151	0.219	0.163
	0.287
0.75ng/ml	0.131	0.095	0.038
	0.058
0ng/ml	0.052	0.056	0
	0.061

Gene ID	989
UniProt ID	Q16181

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104955	E3429Hu	48T	$320		Add to Cart
BHE12104955	E3429Hu	96T	$458		Add to Cart

Human Septin-7 - SEPT7 ELISA Kit

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