Human Semenogelin-1 - SEMG1 ELISA Kit

Mfr.No.

E3266Hu

Description

This sandwich kit is for the accurate quantitative detection of human Semenogelin-1 (also known as SEMG1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Alpha-inhibin-31;Alpha-inhibin-92;Cancer/testis antigen 103;Semenogelin I;Semenogelin-1;SEMG 1;SEMG1;Seminal basic protein;SGI;

Reactivity

Human

Detection Range

0.3-90ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

52131

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.16ng/ml

Background

Predominant protein in semen. It participates in the formation of a gel matrix entrapping the accessory gland secretions and ejaculated spermatozoa. Fragments of semenogelin and/or fragments of the related proteins may contribute to the activation of progressive sperm movements as the gel-forming proteins are fragmented by KLK3/PSA. Alpha-inhibin-92 and alpha-inhibin-31, derived from the proteolytic degradation of semenogelin, inhibit the secretion of pituitary follicle-stimulating hormone.

Gene ID	6406
UniProt ID	P04279
Antigen	SEMG1

Research Area

Cell Biology

Protocol

These standard curves of E3266Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
48ng/ml	2.575	2.625	2.582
	2.674
24ng/ml	1.588	1.576	1.533
	1.564
12ng/ml	0.924	0.924	0.881
	0.924
6ng/ml	0.427	0.44	0.397
	0.453
3ng/ml	0.205	0.241	0.198
	0.277
0ng/ml	0.052	0.043	0
	0.034

Precision

We measured random samples of E3266Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	12.7	0.47	3.7
Intra-Assay	2	18	24.3	0.86	3.5
Intra-Assay	3	18	6.2	0.32	5.2

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104795	E3266Hu	48T	$320		Add to Cart
BHE12104795	E3266Hu	96T	$458		Add to Cart

Human Semenogelin-1 - SEMG1 ELISA Kit

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