Human Semaphorin 6D - SEMA6D ELISA Kit

Mfr.No.

E2537Hu

Sensitivity

4.45ng/L

Detection Range

10-2000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

SEMA6D;Semaphorin-6D

Molecular Weight

119872

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2537Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	522.8	19.75	3.8
Intra-Assay	2	18	681.3	17.25	2.5
Intra-Assay	3	18	385.2	21.37	5.5

Background

Shows growth cone collapsing activity on dorsal root ganglion (DRG) neurons in vitro. May be a stop signal for the DRG neurons in their target areas, and possibly also for other neurons. May also be involved in the maintenance and remodeling of neuronal connections.

Protocol

These standard curves of E2537Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	1.906	2.01	1.974
	2.114
600ng/L	1.139	1.124	1.088
	1.109
300ng/L	0.536	0.562	0.526
	0.588
150ng/L	0.179	0.199	0.163
	0.219
75ng/L	0.123	0.115	0.079
	0.107
0ng/L	0.047	0.036	0
	0.025

Gene ID	80031
UniProt ID	Q8NFY4

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104075	E2537Hu	48T	$320		Add to Cart
BHE12104075	E2537Hu	96T	$458		Add to Cart

Human Semaphorin 6D - SEMA6D ELISA Kit

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